The Anti-Globulin (Coombs) Test in Laboratory Practice

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The production of anti-human globulin serum (Coombs reagent) in goats.

The rabbit is customarily used for the preparation of anti-human globulin serum, but there are several objections to the choice of this animal. These are: (1) The volume of serum obtained is small. To maintain supplies it is necessary either to have several animals producing the reagent, or to hyperimmunize and bleed the rabbits used too frequently, which in turn results in a less specific reag...

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Relationship between post-transplant lymphoproliferative disorder and Anti-Thymocyte Globulin or Anti-Lymphocyte Globulin

 Abstract Background: Lymphoproliferative disorders are among the most serious and potentially fatal complications of chronic immunosuppression in kidney transplant recipients. The principle risk factors for development of PTLD are the degree of overall immunosuppression and the EBV serostatus of the recipient. In this study, the risk of PTLD in kidney transplant recipients who received Anti- L...

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Anti-gamma-globulin consumption test in Hashimoto's disease.

A simple anti-gamma-globulin consumption test for use in suspected Hashimoto's disease is described. Results in cases with positive and negative tanned red-cell tests are described.

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Factors affecting the speed of agglutination in the Coombs test.

The Coombs test (Coombs, Mourant, and Race, 1945a, 1945b, 1946) has become popular as a sensitive and reliable method of detecting incomplete antibodies. The essential reagent is the serum of an animal (usually a rabbit) which has been immunized with human globulin. Potent rabbit sera sometimes show a prozone phenomenon (Wootton, 1950; Van Loghem, Kresner, Coombs, and Fulton Roberts, 1950). The...

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The Direct Antiglobulin (coombs) Test in Megaloblastic Anaemia.

Folic acid deficiency with the picture of a megaloblastic bone marrow may develop in haemolytic anaemia, and, on the other hand, both vitamin B(12) and folic acid deficiency may produce signs of haemolysis. As the correct interpretation of a positive antiglobulin reaction associated with megaloblastic erythropoiesis is particularly important, the effect of deficiency of vitamin B(12) and folic ...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1960

ISSN: 0021-9746

DOI: 10.1136/jcp.13.4.368-a